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Methods in Molecular Biology Ser.: Capillary Electrophoresis of Carbohydrates by Susumu Honda (2010, Trade Paperback)

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A useful appendix describes the structures of the most commonly encountered carbohydrate residues and olgosaccharides from mammalian and bacterial origins. A collection of cutting-edge techniques for using capillary electrophoresis (CE) to analyze complex carbohydrates.

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Glycoconjugates such as glycoproteins and glycolipids play important roles in cell-cell interaction events, including development, differentiation, m- phogenesis, fertilization, inflammation, and metastasis. A number of reports have documented the association of unique oligosaccharide sequences to p- tein targeting and folding, and in mechanisms of infection, inflammation, and immunity. For glycoproteins, these glycan appendages are the result of extensive co- or post-translational modifications of the nascent proteins in the endoplasmic reticulum and in the Golgi apparatus. Although nucleic acids and proteins are copied from a template in a repeated series of identical steps using the same enzymes, complex carbohydrates are formed by the sequential actions of cellular glycosyltransferases that specifically recognize unique s- strates. The molecular biology of these transferases and other carbohydra- modifying enzymes is providing important insights on oligosaccharide recognition events. While it is acknowledged that the definition of the protein complement of cells and tissues (the so-called proteome) remains an enormous task in this postgenomic era, the characterization of all glycans produced by individual organisms (referred to as the glycome) presents an equally imp- tant challenge. This task is further complicated by the fact that oligosacc- rides cannot presently be cloned. These complex carbohydrates exist in a staggering diversity of structures, linkages, and branching, thus providing an exquisite molecular repertoire for cellular interactions.

Product Identifiers

Publisher
Humana Press
ISBN-10
1617372064
ISBN-13
9781617372063
eBay Product ID (ePID)
109324410

Product Key Features

Author
Susumu Honda
Publication Name
Capillary Electrophoresis of Carbohydrates
Format
Trade Paperback
Language
English
Publication Year
2010
Series
Methods in Molecular Biology Ser.
Type
Textbook
Number of Pages
Xiv, 318 Pages

Dimensions

Item Length
9in
Item Width
6in
Item Weight
18 Oz

Additional Product Features

Series Volume Number
213
Number of Volumes
1 Vol.
Lc Classification Number
Qd415-436
Reviews
"...the book will be very valuable for researchers carrying out carbohydrate research, or planning to do so, and contemplating the best analytical approaches." -Pharmaceutical Research"...this book provides a great deal of useful practical detail for CE analysis of carbohydrates for the experienced as well as the newcomer to the field." -Biomedical Chromatography"...the book is a nice compilation of protocols for the analysis of carbohydrates by CE...it does contain all the essential procedures and methods needed to solve many problems." - Chromatographia"...this book comprehensively summarizes knowledge on carbohydrate analysis by means of capillary electrophoresis." - Biochemistry, "...the book will be very valuable for researchers carrying out carbohydrate research, or planning to do so, and contemplating the best analytical approaches." -Pharmaceutical Research "...this book provides a great deal of useful practical detail for CE analysis of carbohydrates for the experienced as well as the newcomer to the field." -Biomedical Chromatography "...the book is a nice compilation of protocols for the analysis of carbohydrates by CE...it does contain all the essential procedures and methods needed to solve many problems." - Chromatographia "...this book comprehensively summarizes knowledge on carbohydrate analysis by means of capillary electrophoresis." - Biochemistry
Table of Content
Structural and Functional Diversity of Glycoconjugates.- Sample Preparation Methods.- Chemical and Enzymatic Release of Glycans from Glycoproteins.- Derivatization of Carbohydrates.- Preparation of Oligosaccharides from Sulfated Glycosaminoglycans Using Bacterial Enzymes.- Analysis of Mono- and Oligosaccharides.- Monosaccharide Analysis by Capillary Electrophoresis.- Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers.- Characterization of Oligosaccharides from Starch, Dextran, Cellulose, and Glycoproteins by Capillary Electrophoresis.- Affinity Capillary Electrophoresis of Oligosaccharides.- Characterization of Glycosaminoglycans by Capillary Electrophoresis.- Analysis of Glycoconjugates and the Component Monosaccharides.- Analysis of 2-Aminoacridone-Derivatized Complex Oligosaccharides Using Micellar Electrokinetic Capillary Chromatography and Laser-Induced Fluorescence Detection.- Separation of Protein Glycoforms by Capillary Electrophoresis.- Separation of Glycoproteins by Capillary Isoelectric Focusing.- Glycoprotein Analysis by Capillary Zone Electrophoresis-Electrospray Mass Spectrometry.- Analysis of Bacterial Glycolipids by Capillary Electrophoresis-Electrospray Mass Spectrometry.- Specific Applications.- Capillary Electrophoresis as an Assay Method for Monitoring Glycosyltransferase Activity.- Determination of Association Constant of Carbohydrate-Protein Interaction.
Copyright Date
2003
Topic
Life Sciences / Biochemistry, Chemistry / Electrochemistry, Chemistry / Organic
Dewey Decimal
572/.56736
Intended Audience
Scholarly & Professional
Dewey Edition
21
Illustrated
Yes
Genre
Science

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Methods in Molecular Biology Ser.: Capillary Electrophoresis of Carbohydrates by Susumu Honda (2010, Trade Paperback)

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