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PCR Troubleshootin
USD251,42
Aproximadamente217,70 EUR
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- ¡Cómpralo ya!PCR Troubleshooting and Optimization: The Essential Guide by Suzanne Kennedy
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Ubicado en: Sparks, Nevada, Estados Unidos
Entrega:
Entrega prevista entre el mié. 18 jun. y el mié. 25 jun. a 94104
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N.º de artículo de eBay:403892802806
Última actualización el 05 feb 2024 12:29:50 H.EspVer todas las actualizacionesVer todas las actualizaciones
Características del artículo
- Estado
- Publication Date
- 2011-01-01
- ISBN
- 9781904455721
Acerca de este producto
Product Identifiers
Publisher
Caister Academic Press
ISBN-10
1904455727
ISBN-13
9781904455721
eBay Product ID (ePID)
84278484
Product Key Features
Topic
Life Sciences / Molecular Biology, Life Sciences / Genetics & Genomics, Research & Methodology
Publication Year
2011
Book Title
Pcr Troubleshooting and Optimization : the Essential Guide
Number of Pages
250 Pages
Language
English
Illustrator
Yes
Genre
Science
Format
Hardcover
Dimensions
Item Weight
22 Oz
Additional Product Features
Dewey Edition
22
Dewey Decimal
572.86
Synopsis
The polymerase chain reaction (PCR) is a fundamental tool in scientific research and clinical testing. Real-time PCR, combining both amplification and detection in one instrument, is a rapid and accurate method for nucleic acid detection and quantification. Although PCR is a very powerful technique, the results achieved are valid only if the appropriate controls have been employed. In addition, proper optimization of PCR conditions is required for the generation of specific, repeatable, reproducible, and sensitive data. This book discusses the strategies for preparing effective controls and standards for PCR, when they should be employed, and how to interpret the information they provide. It highlights the significance of optimization for efficiency, precision, and sensitivity of PCR methodology and provides essential guidance on how to troubleshoot inefficient reactions. Experts in PCR describe design and optimization techniques, discuss the use of appropriate controls, explain the significance of standard curves, and explore the principles and strategies required for effective troubleshooting. The book highlights the importance of sample preparation and quality, primer design, controlling inhibitors, avoiding amplicon and environmental contamination, optimizing reagent quality and concentration, and modifying the thermal cycling protocol for optimal sensitivity and specificity. In addition, specific chapters discuss the history of PCR, the choice of instrumentation, the applications of PCR in metagenomics, high resolution melting analysis, the MIQE guidelines, and PCR at the microliter scale. The strategies, tips and advice contained in this concise volume will enable the scientist to optimize and effectively troubleshoot a wide range of techniques, including PCR, reverse transcriptase PCR, real-time PCR, and quantitative PCR. It will be an essential book for anyone using PCR technology.
LC Classification Number
QP606.D46
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- _***s (6)- Votos emitidos por el comprador.Mes pasadoCompra verificadaWhen I'd originally placed this order, I had accidentally put in the wrong shipping address. However, the seller was more than helpful, and the CD arrived!! The packaging was wide open when it arrived, but luckily nothing was damaged. Additionally, there was nothing protective on the CD, so that open package made me feel kind of nervous. Otherwise, absolutely great! The customer service was awesome, and the product was as described.
- r***w (229)- Votos emitidos por el comprador.Últimos 6 mesesCompra verificadaItem was lost in delivery- but after some communication, seller was understanding and provided a refund. I ordered another and had it sent elsewhere. My main issue was that it felt as if I was getting automated responses at first!! Please have humans review human inquiries!! It was painstaking explaining the situation to an A.I. who only repeats unhelpful replies. Thanks again!! Otherwise. Great price! Great seller!
- e***r (331)- Votos emitidos por el comprador.Mes pasadoCompra verificadaAlthough I received the incorrect book with my order (that had a title close to what I had ordered), AlibrisBooks apologized, said they could not re-send the book, and refunded my money and told me to keep the book that I had received, and either donate it or give it away, so I gave it to someone. That was very kind on their part to do that, and I would highly recommend them. Mistakes happen I get that.
AlibrisBooks
98,5% de votos positivos•1,9 millones artículos vendidos
Registrado como vendedor profesional
Votos de vendedor (507.667)
- _***s (6)- Votos emitidos por el comprador.Mes pasadoCompra verificadaWhen I'd originally placed this order, I had accidentally put in the wrong shipping address. However, the seller was more than helpful, and the CD arrived!! The packaging was wide open when it arrived, but luckily nothing was damaged. Additionally, there was nothing protective on the CD, so that open package made me feel kind of nervous. Otherwise, absolutely great! The customer service was awesome, and the product was as described.
- r***w (229)- Votos emitidos por el comprador.Últimos 6 mesesCompra verificadaItem was lost in delivery- but after some communication, seller was understanding and provided a refund. I ordered another and had it sent elsewhere. My main issue was that it felt as if I was getting automated responses at first!! Please have humans review human inquiries!! It was painstaking explaining the situation to an A.I. who only repeats unhelpful replies. Thanks again!! Otherwise. Great price! Great seller!
- e***r (331)- Votos emitidos por el comprador.Mes pasadoCompra verificadaAlthough I received the incorrect book with my order (that had a title close to what I had ordered), AlibrisBooks apologized, said they could not re-send the book, and refunded my money and told me to keep the book that I had received, and either donate it or give it away, so I gave it to someone. That was very kind on their part to do that, and I would highly recommend them. Mistakes happen I get that.