Digital PCR: Methods and Protocols by George Karlin-Neumann (English) Hardcover

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Características del artículo

Estado
En muy buen estado: Libro que se ha leído y que no tiene un aspecto nuevo, pero que está en un ...
Book Title
Digital PCR
ISBN-13
9781493977765
ISBN
9781493977765
Categoría

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Product Identifiers

Publisher
Springer New York
ISBN-10
1493977768
ISBN-13
9781493977765
eBay Product ID (ePID)
9038382819

Product Key Features

Number of Pages
Xv, 557 Pages
Language
English
Publication Name
Digital Pcr : Methods and Protocols
Subject
Life Sciences / Molecular Biology, Genetics
Publication Year
2018
Type
Textbook
Author
Francisco Bizouarn
Subject Area
Science, Medical
Series
Methods in Molecular Biology Ser.
Format
Hardcover

Dimensions

Item Weight
45.3 Oz
Item Length
10 in
Item Width
7 in

Additional Product Features

Reviews
"The editors of the volume have succeeded in providing specialists in different sectors with a wide range of technology applications that can be adapted to other platforms or can provide the basis for creating new ones." (Paolo Catarsi, European Journal of Histochemistry, Vol. 63, 2019)
Series Volume Number
1768
Number of Volumes
1 vol.
Illustrated
Yes
Table Of Content
Entering the Pantheon of 21st Century Molecular Biology Tools: A Perspective on Digital PCR.- Basic Concepts and Validation of Digital PCR Measurements.- Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies, What Does it Mean?.- Control Materials and Digital PCR Methods for Evaluation of Circulating Cell Free DNA Extractions from Plasma.- Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events.- Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples.- Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR.- Testing of General and Human-Associated Fecal Contamination in Waters.- Analyzing Copy Number Variation with Droplet Digital PCR.- Assessing HER2 Amplification in Plasma cfDNA.- Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues using ddPCR: analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants.- Monitoring of Response and Resistance in Plasma of EGFR-Mutant Lung Cancer using Droplet Digital PCR.- Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients.- Droplet Digitial PCR for Miniaml Residual Disease Detection in Mature Lymphoproliferative Disorders.- Quantitation of JAK2 V617F Allele Burden by using the QuantStudio(tm) 3D Digitial PCR System.- Novel Multiplexing Strategies for Quantification of Rare Alleles using ddPCR.- Identification and Use of Personalized Genomic Markers for Monitoring Circulating Tumor DNA.- Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant Genotyping.- A Universal Droplet Digital PCR Approach for Monitoring of Graft Health after Transplantation using a Pre-Selected SNP Set.- Detection and Quantification of HDR and NHEJ Induced by Genome Editing at Endogenous Gene Loci using Droplet Digital PCR.- DNA Methylation Analysis Using Droplet Digital PCR.- Simultaneous Quantification of Multiple Alternatively Spliced mRNA Transcripts using Droplet Digital PCR.- Using Droplet Digital PCR to Analyze Allele-Specific RNA Expression.- Very Low Abundance Single-Cell Transcript Quantification with 5-Plex ddPCR(tm) Assays.- Quantification of Circulating MicroRNAs by Droplet Digital PCR using EvaGreen Probeless Chemistry.- Droplet Digital PCR for Absolute Quantification of Extracellular MicroRNAs in Plasma and Serum: Quantification of the Cancer Biomarker has-miR-141.- Droplet Digital(tm) PCR Next-Generation Sequencing Library QC Assay.- Phasing DNA Markers using Digital PCR.- ddTRAP: A Method for Sensitive and Precise Quantification of Telomerase Activity.- Highly Efficient and Reliable DNA Aptamer Selection Using the Partitioning Capabilities of ddPCR: The Hi-Fi SELEX Method.
Synopsis
Preface...Table of Contents...Contributing Authors... Part I Background Concepts 1. Entering the Pantheon of 21st Century Molecular Biology Tools: A Perspective on Digital PCRGeorge Karlin-Neumann and Frank Bizouarn 2. Basic Concepts and Validation of Digital PCR MeasurementsLeonardo Pinheiro and Kerry R. Emslie 3. Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies, What Does it Mean?Svilen Tzonev 4. Control Materials and Digital PCR Methods for Evaluation of Circulating Cell Free DNA Extractions from PlasmaAlexandra S. Whale, Ana Fernandez-Gonzalez, Alice Gutteridge, and Alison S. Devonshire Part II Absolute Quantification 5. Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize EventsDavid Dobnik, Bj rn Spilsberg, Alexandra Bogozalec, Dejan Stebih, Dany Morisset, Arne Holst-Jensen and Jana Zel 6. Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical SamplesAshlet Vellucci, Emily Leibovitch, and Steven Jacobson 7. Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCRPetar Podlesniy and Ramon Trullas 8. Testing of General and Human-Associated Fecal Contamination in WatersYiping Cao, Mereditch R. Raith, and John F. Griffith Part III Copy Number Variation 9. Analyzing Copy Number Variation with Droplet Digital PCRAvery Davis Bell, Christina L. Usher, and Steven A. McCarroll 10. Assessing HER2 Amplification in Plasma cfDNAIsaac Garcia-Murillas and Nick Turner 11. Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues using ddPCR: Analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide VariantsBo Zhou, Michael S. Haney, Xiaowei Zhu, Reenal Pattni, Alexej Abyzov, and Alexander E. Urban Part IV Rare Mutation and Rare Allele Detection 12. Monitoring of Response and Resistance in Plasma of EGFR-Mutant Lung Cancer using Droplet Digital PCRYanan Kuang, Adrian G. Sacher, Allison O'Connell, Nora Feeney, Ryan S. Alden, Geoffrey R. Oxnard, and Cloud P. Paweletz 13. Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer PatientsArielle J. Medford, Riaz N. Gillani, and Ben Ho Park 14. Droplet Digitial PCR for Miniaml Residual Disease Detection in Mature Lymphoproliferative DisordersDaniela Drandi, Simone Ferrero, and Marco Ladetto 15. Quantitation of JAK2 V617F Allele Burden by using the QuantStudio(TM) 3D Digitial PCR SystemElena Kinz and Axel Muendlein 16. Novel Multiplexing Strategies for Quantification of Rare Alleles using ddPCRMiguel Alcaide and Ryan Morin 17. Identification and Use of Personalized Genomic Markers for Monitoring Circulating Tumor DNAYilun Chen, Anthony George, Eleonor Olsson, and Lao H. Saal 18. Single Color Multiplexed ddPCR Copy Number Measurements and Single Nucleotide Variant GenotypingChristina Wood and Hanlee P. Ji 19. A Universal Droplet Digital PCR Approach for Monitoring of Graft Health after Transplantation using a Pre-Selected SNP SetJulia Beck, Michael Oellerich, and Ekkehard Schütz 20. Detection and Quantification of HDR and NHEJ Induced by Genome Editi, This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Digital PCR: Methods and Protocols is a valuable resource for specialists in various fields, including geneticists, neurologists, immunologists, oncologists, and researchers who are interested in environmental sciences."
LC Classification Number
RB155-155.8

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