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Methods in Molecular Biology Ser.: Basic Protein and Peptide Protocols by Anthony Walker (1994, Hardcover)
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10), Practical Protein Chro- tography (vol. 14), Protein-DNA Interactions (vol. 17), and Optical Spectroscopy, Microscopy, and Macroscopic Techniques (vol.
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Product Identifiers
PublisherHumana Press
ISBN-10089603268X
ISBN-139780896032682
eBay Product ID (ePID)5038439450
Product Key Features
Number of PagesXii, 490 Pages
LanguageEnglish
Publication NameBasic Protein and Peptide Protocols
SubjectLife Sciences / Biochemistry, Chemistry / Organic
Publication Year1994
TypeTextbook
AuthorAnthony Walker
Subject AreaScience
SeriesMethods in Molecular Biology Ser.
FormatHardcover
Dimensions
Item Weight68.4 Oz
Item Length9.3 in
Item Width6.1 in
Additional Product Features
Intended AudienceScholarly & Professional
LCCN93-048929
Reviews...would I buy this volume? I think that the answer to this is yes. It is a useful and concise volume that would be of great value around the lab. I would also recommend it for libraries as it provides an excellent reference source on techniques of protein analysis for undergraduates entering the difficult world of research projects. The value of this volume lies in the fact that any potential reader is liable to use the techniques described in several chapters to pursue a piece of research work. - Trends in Biochemical Sciences
Dewey Edition20
Series Volume Number32
Number of Volumes1 vol.
IllustratedYes
Dewey Decimal574.19/245
Table Of ContentThe Lowry Method for Protein Quantitation.- The Bicinchoninic Acid (BCA) Assay for Protein Quantitation.- The Bradford Method for Protein Quantitation.- Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins.- SDS Polyacrylamide Gel Electrophoresis of Proteins.- Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins.- Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Proteins.- Electrophoresis of Peptides.- Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels.- The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis.- Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins.- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dimension.- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension.- Quantification of Proteins on Polyacrylamide Gels (Nonradioactive).- Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique.- Identification of Glycoproteins on Nitrocellulose Membranes and Gels.- The Release of Oligosaccharides from Glycoproteins.- Structural Profiling of Oligosaccharides of Glycoproteins.- Drying Gels.- Fluorography of Polyacrylamide Gels Containing Tritium.- The Electrophoretic Elution of Proteins from Polyacrylamide Gels.- Preparation of Proteins from Gels for Protein Microsequencing.- Comparison of Protein Primary Structures.- Protein Blotting.- Detection of Polypeptides on Immunoblots Using Secondary Antibodies or Protein A.- Detection of Proteins on Blots Using the Avidin-Biotin System.- Detection of Proteins on Protein Blots Using Chemiluminescent Systems.- The Detection of Proteins on Blots Using Gold or Immunogold.- Amino Acid Analysis.- Molecular-Weight Estimation for Native Proteins Using Size-Exclusion High-Performance Liquid Chromatography.- Peptide Purification by Reverse-Phase HPLC.- Enzymatic Methods for Cleaving Proteins.- Chemical Cleavage of Proteins.- Chemical Modification of Proteins.- The Dansyl Method for Identifying N-Terminal Amino Acids.- The Dansyl-Edman Method for Peptide Sequencing.- C-Terminal Sequencing of Peptides.- Analysis of Cysteine Residues and Disulfide Bonds.- Preparation and Characterization of Monoclonal Antibodies to Proteins and Other Cellular Components.- The Raising of a Polyclonal Antiserum to a Protein.- Production of Antisera to Synthetic Peptides.- Production of Antibodies Using Proteins in Gel Bands.- Preparation of Purified Immunoglobulin G (IgG).- Enzyme and Biotin Labeling of Antibody.- Labeling of Peptides and Proteins by Radioiodination.- Radioimmunoassay of Peptides and Proteins.- Enzyme-Linked Immunosorbent Assay (ELISA).- Free Zone Capillary Electrophoresis.
SynopsisThe first volume in this Methods Molecular Biology series, Proteins (1984), concentrated on basic techniques for the analysis and purification of peptides and proteins. As the series developed, more specialized volumes on proteins were introduced, such as those on Immunochemical Protocols (vol. 10), Practical Protein Chro- tography (vol. 11), Analysis Glycoprotein Biomedicine (vol. 14), Protein-DNA Interactions (vol. 30), Biomembrane Protocols (vols. 19 and 27), Analyses and Methods (vol. 17), and Optical Spectroscopy, Microscopy, and Macroscopic Techniques (vol. 22). Further specialist volumes on peptides, monoclonal antibodies, immunoassays, ELISA, protein engineering, protein stability, mass spectrometry of proteins, automated sequence analysis, and protein NMR are currently in preparation. Since it is now a decade since the initial volume was published, it seems an especially appropriate moment to extensively reorganize, update, and revise the earlier volume. In an attempt to be more c- prehensive in our coverage, this current volume, Basic Protein and Peptide Protocols, is totally committed to basic analytical methods; a planned companion volume will later concentrate on preparative techniques. Those analytical techniques requiring expensive speci- ized instrumentation, such as NMR, mass spectrometry, X-ray cr- tallography, spectroscopy, and automated sequence analysis, are not described here, but in the appropriate specialized volumes listed above., Basic Protein and Peptide Protocols offers an excellent collection of reproducible step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins (2) the electrophoretic and blotting procedures used in protein isolation and characterization and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!
LC Classification NumberQD415-436
